Can I use the Twist kits to make libraries for Whole Genome Sequencing?

Yes, the Twist Library Prep kits are suitable to make libraries for whole genome sequencing.

As most WGS applications use longer read lengths (2x151 for Whole Genome Sequencing as opposed to 2x101 or 2x76 for Whole Exome Sequencing), the fragmentation conditions need to be changed to accommodate longer fragment lengths.

For the Enzymatic Fragmentation kits, length is dependent on three factors: Time, Temperature, and DNA input.

For Mechanical fragmentation, conditions (time, power, duty factor, and cycles per burst) should to be set to accommodate longer fragment lengths.

 

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