Eliminate Sequence Bias and Undesirable Motifs
Eliminate Sequence Bias and Undesirable Motifs
Eliminate Sequence Bias and Undesirable Motifs
OVERVIEW DATA ORDERING AND SPECS RESOURCES
Overview

Precision Variant Library Technology Allows for Focused Screening

Twist’s massively parallel silicon-based DNA synthesis platform produces highly uniform and accurate oligos, with 90% of oligos represented within <2.5x of the mean, along with an industry-leading low error rate of 1:2,000 nt.

Combined with our well established molecular biology expertise, the Twist oligo synthesis platform enables the fabrication of highly diverse gene mutant libraries with excellent variant representation and highly specific user-defined composition with no unwanted bias or motifs. Twist library technology enables a comprehensive interrogation of the variant sequence space.

High Diversity Precision
High Diversity Precision
Effortlessly incorporate multiple variant domains into one or multiple scaffolds
Precise control over codon usage (all 64 codons), amino acid distribution, and length variation
Verified Quality
Verified Quality
Rigorous quality control, including NGS verification of modified regions
Sequence variant ratios documented
Uniform distribution of user-defined variants
No premature stop codons or unwanted codons
Flexibility
Flexibility
Design all sequences, single or multiple domains and combinations— single, pairwise, or triple variants
Modular synthesis system enables iteration of future libraries
Greater flexibility than TRIM and the degenerate approach
Precision Variant Library Technology Allows for Focused Screening

Learn more about Twist Bioscience’s ability to construct complex, high diversity libraries at confined regions (for example, CDRs) and introduce technology advancements that enable the construction of synthetic DNA libraries with diversity scattered along the length of constructs.

Watch the Webinar

Precision Variant Library Technology Allows for Focused Screening

Twist’s massively parallel silicon-based DNA synthesis platform produces highly uniform and accurate oligos, with 90% of oligos represented within <2.5x of the mean, along with an industry-leading low error rate of 1:2,000 nt.

Combined with our well established molecular biology expertise, the Twist oligo synthesis platform enables the fabrication of highly diverse gene mutant libraries with excellent variant representation and highly specific user-defined composition with no unwanted bias or motifs. Twist library technology enables a comprehensive interrogation of the variant sequence space.

High Diversity Precision
High Diversity Precision
Effortlessly incorporate multiple variant domains into one or multiple scaffolds
Precise control over codon usage (all 64 codons), amino acid distribution, and length variation
Verified Quality
Verified Quality
Rigorous quality control, including NGS verification of modified regions
Sequence variant ratios documented
Uniform distribution of user-defined variants
No premature stop codons or unwanted codons
Flexibility
Flexibility
Design all sequences, single or multiple domains and combinations— single, pairwise, or triple variants
Modular synthesis system enables iteration of future libraries
Greater flexibility than TRIM and the degenerate approach
Precision Variant Library Technology Allows for Focused Screening

Learn more about Twist Bioscience’s ability to construct complex, high diversity libraries at confined regions (for example, CDRs) and introduce technology advancements that enable the construction of synthetic DNA libraries with diversity scattered along the length of constructs.

Watch the Webinar

DATA
High Quality Data

Twist Bioscience’s silicon-based DNA synthesis platform and library technology provides scientists with high-quality libraries that produce reliable data in less time. When compared to two other competing technologies (figure 1), Twist’s library showed less than 1% deviation from the designed amino acid frequency.  In addition to precisely matching designed amino acid ratios, Twist’s in-silico DNA synthesis platform seamlessly incorporates desirable binding motifs and length variation across multi-domain libraries, giving scientists the power to precisely design and customize variant libraries that enable a comprehensive analysis of the variant space.

And with Twist libraries, the problems and challenges typical of NNK and TRIM libraries are avoided. Each variant is printed base-by-base and screened prior to synthesis, eliminating stop codons, liability motifs, unwanted mutations, and any undesirable biases, all at the beginning of the process. As a result, the library is enriched for the requested functional variants and the screening burden is reduced..

Our industry-leading, ready to use, highly-diverse and precisely designed libraries give scientists more opportunities to achieve their research goals.

Twist Technology Comparison NNK vs Trimer vs Twist
Superior Libraries

At Twist, we use molecular biology expertise to precisely construct variant libraries. Our single-base control approach allows us to deliver high-diversity libraries without motifs that could confound your screening process. We deliver fully-customized libraries of unparalleled quality, with desired variants present at user-defined rations. Here you can see a CVL example representative of that quality. Variants in seven sequential amino acid positions were generated and all have expected variants at the positions shown, with nearly all at the desired ratio:

At position 1 and 6, the wild type amino acid was requested at 40% (position 1) and 30% (position 7). The remaining 18 amino acids were all requested to be low as 3.3%.

At position 3-5 amino acid residues were requested and observed at 5.3%.

Variant Libraries CVL Distribution
User-Defined CDR Libraries

Our Precise Variant Libraries allow you to choose what unique CDR (complimentary defining regions) sequences you want to be incorporated into the choice of framework(s).

Each CDR can be codon-optimized to avoid the creation of unwanted restriction sites. Machine learning has become an integral part of scientific research and has been used as a tool to analyze antibody libraries and identify unique CDR combinations that would yield, for example, higher affinity and specificity. 

Coupled with Twist’s silicon-based synthesis platform, explicit library combinations generated from the analysis can be synthesized and seamlessly incorporated into a fully synthetic library to refine the exploration of the variant space.

User-Defined CDR Libraries

 

Libraries QC’d with NGS

Since every Libraries verified with NGS, negative data can be used to identify mutations that do not yield improved functions, and those can be removed in the next iteration of library design.

The table shows the amino acid frequency (%) at seven sites of a mutagenized region synthesized using. Variants in seven sequential amino acid positions were generated with 19 amino acid residues (cysteine was omitted) in the first seven sites. A tabulated frequency data obtained from NGS, with the shade of green indicating deviation from the expected value. All expected variants were present at all positions and their observed frequency was within 25% of the expected value (specification) at 5.3%.

Variant Libraries CVL Distribution
High Quality Data

Twist Bioscience’s silicon-based DNA synthesis platform and library technology provides scientists with high-quality libraries that produce reliable data in less time. When compared to two other competing technologies (figure 1), Twist’s library showed less than 1% deviation from the designed amino acid frequency.  In addition to precisely matching designed amino acid ratios, Twist’s in-silico DNA synthesis platform seamlessly incorporates desirable binding motifs and length variation across multi-domain libraries, giving scientists the power to precisely design and customize variant libraries that enable a comprehensive analysis of the variant space.

And with Twist libraries, the problems and challenges typical of NNK and TRIM libraries are avoided. Each variant is printed base-by-base and screened prior to synthesis, eliminating stop codons, liability motifs, unwanted mutations, and any undesirable biases, all at the beginning of the process. As a result, the library is enriched for the requested functional variants and the screening burden is reduced..

Our industry-leading, ready to use, highly-diverse and precisely designed libraries give scientists more opportunities to achieve their research goals.

Twist Technology Comparison NNK vs Trimer vs Twist
Superior Libraries

At Twist, we use molecular biology expertise to precisely construct variant libraries. Our single-base control approach allows us to deliver high-diversity libraries without motifs that could confound your screening process. We deliver fully-customized libraries of unparalleled quality, with desired variants present at user-defined rations. Here you can see a CVL example representative of that quality. Variants in seven sequential amino acid positions were generated and all have expected variants at the positions shown, with nearly all at the desired ratio:

At position 1 and 6, the wild type amino acid was requested at 40% (position 1) and 30% (position 7). The remaining 18 amino acids were all requested to be low as 3.3%.

At position 3-5 amino acid residues were requested and observed at 5.3%.

Variant Libraries CVL Distribution
User-Defined CDR Libraries

Our Precise Variant Libraries allow you to choose what unique CDR (complimentary defining regions) sequences you want to be incorporated into the choice of framework(s).

Each CDR can be codon-optimized to avoid the creation of unwanted restriction sites. Machine learning has become an integral part of scientific research and has been used as a tool to analyze antibody libraries and identify unique CDR combinations that would yield, for example, higher affinity and specificity. 

Coupled with Twist’s silicon-based synthesis platform, explicit library combinations generated from the analysis can be synthesized and seamlessly incorporated into a fully synthetic library to refine the exploration of the variant space.

User-Defined CDR Libraries

 

Libraries QC’d with NGS

Since every Libraries verified with NGS, negative data can be used to identify mutations that do not yield improved functions, and those can be removed in the next iteration of library design.

The table shows the amino acid frequency (%) at seven sites of a mutagenized region synthesized using. Variants in seven sequential amino acid positions were generated with 19 amino acid residues (cysteine was omitted) in the first seven sites. A tabulated frequency data obtained from NGS, with the shade of green indicating deviation from the expected value. All expected variants were present at all positions and their observed frequency was within 25% of the expected value (specification) at 5.3%.

Variant Libraries CVL Distribution
Ordering and Specs

We look forward to helping you place your order for your Site Saturation Variant Libraries. Please click on the "Get Quote" button above, where you will be directed to complete a short form and someone will be in contact with you shortly to help complete your order. 

If you already have an account set up with Twist, you can log in and start completing your order details on-line.
 

We look forward to helping you place your order for your Site Saturation Variant Libraries. Please click on the "Get Quote" button above, where you will be directed to complete a short form and someone will be in contact with you shortly to help complete your order. 

If you already have an account set up with Twist, you can log in and start completing your order details on-line.
 

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