NGS Library Preparation Kits

More diversity, More discovery

OVERVIEW WORKFLOW ORDERING & SPECS RESOURCES

OVERVIEW

Twist Library Preparation Kits streamline the construction of high-quality DNA libraries for next-generation sequencing (NGS) applications. Two configurations accommodate either enzymatic or mechanical DNA fragmentation, and both are optimized to facilitate library preparation for whole genome sequencing and targeted enrichment. The kits combine library preparation steps into a single reaction for improved efficiency and consistent results.

Available configurations

Mechanical Fragmentation

This kit is designed for use with gDNA that has already been sheared mechanically. It combines the enzymes and reagents for end repair, dA-tailing, and adapter ligation into a single reaction.

Accommodates a wide range of DNA input types
Enables high-quality sequencing of low-quality samples
Minimizes start / stop site artifacts

Enzymatic Fragmentation

This kit incorporates the reagents and enzymes needed for DNA fragmentation with those for end repair, dA-tailing, and adapter ligation into a single reaction.

Ideal for automated, high-throughput library preparation
Produces tunable, reproducible fragment sizes
Minimizes sequence bias, maximizes coverage depth

Overview of workflow application options

Based on your application needs, create sequencing ready libraries in as little as two hours.

NGS Library Preparation Workflow

KEY BENEFITS

Accommodate Either Enzymatic or Mechanical Fragmentation

Use enzymatic fragmentation for automated, high-throughput gDNA

Use mechanical fragmentation for DNA input of varying types and quality

Accommodate Either Whole Genome Sequencing or Targeted Enrichment

Use with Twist full-length CD adapters for PCR-free library Preparation

Use with the Twist Universal Adapter (UDI) System for maximum yield

Streamline Your Workflow

Optimized for use in the Twist Targeted NGS workflow

Flexible DNA sample input from 1 ng – 1 µg (enzymatic)

Convert DNA samples into robust, amplified libraries in under 2.5 hours

Combine library preparation steps into a single reaction for improved efficiency and consistent results

DOWNLOADS

Twist NGS Library Preparation Kits Product Sheet

Protocol Mechanical Fragmentation and Twist Universal Adapter System: For use with the Twist NGS Workflow

Protocol Enzymatic Fragmentation and Twist Universal Adapter System: For use with the Twist NGS Workflow

Available configurations

Mechanical Fragmentation

This kit is designed for use with gDNA that has already been sheared mechanically. It combines the enzymes and reagents for end repair, dA-tailing, and adapter ligation into a single reaction.

Accommodates a wide range of DNA input types
Enables high-quality sequencing of low-quality samples
Minimizes start / stop site artifacts

Enzymatic Fragmentation

This kit incorporates the reagents and enzymes needed for DNA fragmentation with those for end repair, dA-tailing, and adapter ligation into a single reaction.

Ideal for automated, high-throughput library preparation
Produces tunable, reproducible fragment sizes
Minimizes sequence bias, maximizes coverage depth

Overview of workflow application options

Based on your application needs, create sequencing ready libraries in as little as two hours.

NGS Library Preparation Workflow

KEY BENEFITS

Accommodate Either Enzymatic or Mechanical Fragmentation

Use enzymatic fragmentation for automated, high-throughput gDNA

Use mechanical fragmentation for DNA input of varying types and quality

Accommodate Either Whole Genome Sequencing or Targeted Enrichment

Use with Twist full-length CD adapters for PCR-free library Preparation

Use with the Twist Universal Adapter (UDI) System for maximum yield

Streamline Your Workflow

Optimized for use in the Twist Targeted NGS workflow

Flexible DNA sample input from 1 ng – 1 µg (enzymatic)

Convert DNA samples into robust, amplified libraries in under 2.5 hours

Combine library preparation steps into a single reaction for improved efficiency and consistent results

DOWNLOADS

Twist NGS Library Preparation Kits Product Sheet

Protocol Mechanical Fragmentation and Twist Universal Adapter System: For use with the Twist NGS Workflow

Protocol Enzymatic Fragmentation and Twist Universal Adapter System: For use with the Twist NGS Workflow

WORKFLOW

Overview of the library preparation workflow

Both Twist Library Preparation Kits include the reagents required for end repair, dA-tailing, adapter ligation and library amplification. The Enzymatic Fragmentation Kit also incorporates the enzymes for fragmentation of gDNA samples. Following core library construction, either full length or universal adapters can be used to suit your application needs.

The chart to the right shows how using Twist Universal Adapters for insert ligation and UDI primer PCR amplification generates a robust library.

Overview of the library preparation workflow

The chart to the right shows how using Twist Universal Adapters for insert ligation and UDI primer PCR amplification generates a robust library.

ORDERING & SPECS

PRODUCT SKUS – ENZYMATIC FRAGMENTATION 1.0

104174

Twist Library Preparation EF Kit 1.0, Enzymatic Fragmentation, 16 Samples

104175

Twist Library Preparation EF Kit 1.0, Enzymatic Fragmentation, 96 Samples

PRODUCT SKUS – MECHANICAL FRAGMENTATION WITH AMPLIFICATION MIX

104176

Twist Library Preparation Kit, Mechanical Fragmentation, 16 Samples

104177

Twist Library Preparation Kit, Mechanical Fragmentation, 96 Samples

The Twist NGS Library Preparation Kits are for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.

PRODUCT SKUS - ENZYMATIC FRAGMENTATION 2.0

104206

Twist Library Preparation EF Kit 2.0, 16 Samples

104207

Twist Library Preparation EF Kit 2.0, 96 Samples