Twist Library Preparation Kits streamline the construction of high-quality DNA libraries for next-generation sequencing (NGS) applications. Two configurations accommodate either enzymatic or mechanical DNA fragmentation, and both are optimized to facilitate library preparation for whole genome sequencing and targeted enrichment. The kits combine library preparation steps into a single reaction for improved efficiency and consistent results.
Available configurations
Mechanical Fragmentation
This kit is designed for use with gDNA that has already been sheared mechanically. It combines the enzymes and reagents for end repair, dA-tailing, and adapter ligation into a single reaction.
- Accommodates a wide range of DNA input types
- Enables high-quality sequencing of low-quality samples
- Minimizes start / stop site artifacts
Enzymatic Fragmentation
This kit incorporates the reagents and enzymes needed for DNA fragmentation with those for end repair, dA-tailing, and adapter ligation into a single reaction.
- Ideal for automated, high-throughput library preparation
- Produces tunable, reproducible fragment sizes
- Minimizes sequence bias, maximizes coverage depth
Overview of workflow application options
Based on your application needs, create sequencing ready libraries in as little as two hours.
Twist Library Preparation Kits streamline the construction of high-quality DNA libraries for next-generation sequencing (NGS) applications. Two configurations accommodate either enzymatic or mechanical DNA fragmentation, and both are optimized to facilitate library preparation for whole genome sequencing and targeted enrichment. The kits combine library preparation steps into a single reaction for improved efficiency and consistent results.
Available configurations
Mechanical Fragmentation
This kit is designed for use with gDNA that has already been sheared mechanically. It combines the enzymes and reagents for end repair, dA-tailing, and adapter ligation into a single reaction.
- Accommodates a wide range of DNA input types
- Enables high-quality sequencing of low-quality samples
- Minimizes start / stop site artifacts
Enzymatic Fragmentation
This kit incorporates the reagents and enzymes needed for DNA fragmentation with those for end repair, dA-tailing, and adapter ligation into a single reaction.
- Ideal for automated, high-throughput library preparation
- Produces tunable, reproducible fragment sizes
- Minimizes sequence bias, maximizes coverage depth
Overview of workflow application options
Based on your application needs, create sequencing ready libraries in as little as two hours.
Both Twist Library Preparation Kits include the reagents required for end repair, dA-tailing, adapter ligation and library amplification. The Enzymatic Fragmentation Kit also incorporates the enzymes for fragmentation of gDNA samples. Following core library construction, either full length or universal adapters can be used to suit your application needs.
The chart to the right shows how using Twist Universal Adapters for insert ligation and UDI primer PCR amplification generates a robust library.
Both Twist Library Preparation Kits include the reagents required for end repair, dA-tailing, adapter ligation and library amplification. The Enzymatic Fragmentation Kit also incorporates the enzymes for fragmentation of gDNA samples. Following core library construction, either full length or universal adapters can be used to suit your application needs.
The chart to the right shows how using Twist Universal Adapters for insert ligation and UDI primer PCR amplification generates a robust library.
The Twist NGS Library Preparation Kits are for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.
The Twist NGS Library Preparation Kits are for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.
Protocol
Mechanical Fragmentation and Twist Universal Adapter System: For use with the Twist NGS Workflow
Protocol
Enzymatic Fragmentation and Twist Universal Adapter System: For use with the Twist NGS Workflow
Protocol
Mechanical Fragmentation and Twist Universal Adapter System: For use with the Twist NGS Workflow
Protocol
Enzymatic Fragmentation and Twist Universal Adapter System: For use with the Twist NGS Workflow
