The Twist Universal Adapter System improves the overall performance of any ‘T-A’ overhang ligation library preparation workflow for next-generation sequencing (NGS) applications. Engineered to optimize the performance of Twist Target Enrichment products, the system consists of:
Twist Universal Adapters
Universal TruSeq-compatible adapters with a proprietary design that are compatible with all ‘T-A’ overhang workflows, eliminate formation of undesired adapter dimer, and contribute to increasing overall yield of the workflow.
Twist 10 bp Unique Dual-Indexed (UDI) Primers
primers designed with unique dual indices for each mixed primer pair; provided in a 96-well plate format suitable for automation applications. Index design allows for correction of substitutions, insertions, and deletions within the index reads during post-sequencing processing.
The Twist Universal Adapter System improves the overall performance of any ‘T-A’ overhang ligation library preparation workflow for next-generation sequencing (NGS) applications. Engineered to optimize the performance of Twist Target Enrichment products, the system consists of:
Twist Universal Adapters
Universal TruSeq-compatible adapters with a proprietary design that are compatible with all ‘T-A’ overhang workflows, eliminate formation of undesired adapter dimer, and contribute to increasing overall yield of the workflow.
Twist 10 bp Unique Dual-Indexed (UDI) Primers
primers designed with unique dual indices for each mixed primer pair; provided in a 96-well plate format suitable for automation applications. Index design allows for correction of substitutions, insertions, and deletions within the index reads during post-sequencing processing.
When used with Twist 10 bp UDI Primers, Twist Universal Adapters return higher total yields with fewer PCR cycles. This ultimately leads to the production of more library with fewer PCR errors to drive improvements in target enrichment efficiency.
The chart to the right shows how using Twist Universal Adapters for insert ligation and UDI primer PCR amplification generates a robust library.
Library preparation and target enrichment work together to influence the ability to call variants in sequencing. Consistently constructing robust libraries is important for successful hybrid capture. When adapters dimerize during library preparation, they generate undesired products and reduce the effective concentration of adapters available for ligation to inserts. Using Twist Universal Adapters and Primers during library construction minimizes this problem.
Twist Universal Adapters minimize adapter dimer formation during library preparation.
When used with Twist 10 bp UDI Primers, Twist Universal Adapters return higher total yields with fewer PCR cycles. This ultimately leads to the production of more library with fewer PCR errors to drive improvements in target enrichment efficiency.
The chart to the right shows how using Twist Universal Adapters for insert ligation and UDI primer PCR amplification generates a robust library.
Library preparation and target enrichment work together to influence the ability to call variants in sequencing. Consistently constructing robust libraries is important for successful hybrid capture. When adapters dimerize during library preparation, they generate undesired products and reduce the effective concentration of adapters available for ligation to inserts. Using Twist Universal Adapters and Primers during library construction minimizes this problem.
Twist Universal Adapters minimize adapter dimer formation during library preparation.
The Twist Universal Adapter Systeml is for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.
The Twist Universal Adapter Systeml is for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.
