A New Gold Standard for Epigenetic Analysis
A New Gold Standard for Epigenetic Analysis
A New Gold Standard for
Epigenetic Analysis
OVERVIEW LIBRARY PREP TARGET ENRICHMENT ORDERING RESOURCES
OVERVIEW

The Twist NGS Methylation Detection System provides a robust, end-to-end sample preparation solution for identifying methylated regions in the human genome. The workflow employs a unique enzymatic process from New England Biolabs® that is much less damaging to DNA, alongside Twist‘s Custom Methylation Panel design.

Whether you are investigating cellular differentiation or screening liquid biopsies for cancer, the system offers the most efficient methylation detection available.
 

 

An End-to-End Solution
An End-to-End Solution
State-of-the-art enzymatic conversion of unmethylated cytosines
Uniform capture with high-performance custom Twist probes
Improve on-target rates with optimized reagents
Innovative Library Preparation
Innovative Library Preparation
Better results from higher library complexity and better GC balances
Detects 15% more CpGs than bisulfite conversion
Less sample damage enables challenging sample inputs
Easily drop the workflow into existing Methyl-seq pipelines
temp
Optimized Hybridization Reagents
Adjust hybridization timing without sacrificing performance
Improved on-target rates with Methylation Enhancer
Hybridization in less than 4 hours
Highly Efficient Custom Panels*
Highly Efficient Custom Panels*
Sophisticated design, accurate synthesis, and detailed QC maximize capture uniformity and reproducibility
Capture sense, antisense, methylated and unmethylated DNA simultaneously
Outstanding performance across panel sizes, target regions, and multiplexing requirements
Easily add or enhance panel content

*sold seperately

Unlocking the Complexity of Methylation

Hear from the Twist Product Manager and an NEB Application Scientist as they provide an overview of the NEB-to-Twist workflow. Learn the advantages of enzymatic conversion, the benefits of the Twist pre-capture conversion probe design approach, and the workflow's applicability to clinically relevant sample types.

Watch the Webinar 
 

 

Tools to Improve Methyl-seq Efficiency for Better Resolution in Epigenetic Studies

Dive deeper into Twist Bioscience's new Methyl-seq workflow built in partnership with New England Biolabs. Learn how it leads to increased confidence in methylated-base calls.

Read the Blog

The Twist NGS Methylation Detection System provides a robust, end-to-end sample preparation solution for identifying methylated regions in the human genome. The workflow employs a unique enzymatic process from New England Biolabs® that is much less damaging to DNA, alongside Twist‘s Custom Methylation Panel design.

Whether you are investigating cellular differentiation or screening liquid biopsies for cancer, the system offers the most efficient methylation detection available.
 

 

An End-to-End Solution
An End-to-End Solution
State-of-the-art enzymatic conversion of unmethylated cytosines
Uniform capture with high-performance custom Twist probes
Improve on-target rates with optimized reagents
Innovative Library Preparation
Innovative Library Preparation
Better results from higher library complexity and better GC balances
Detects 15% more CpGs than bisulfite conversion
Less sample damage enables challenging sample inputs
Easily drop the workflow into existing Methyl-seq pipelines
temp
Optimized Hybridization Reagents
Adjust hybridization timing without sacrificing performance
Improved on-target rates with Methylation Enhancer
Hybridization in less than 4 hours
Highly Efficient Custom Panels*
Highly Efficient Custom Panels*
Sophisticated design, accurate synthesis, and detailed QC maximize capture uniformity and reproducibility
Capture sense, antisense, methylated and unmethylated DNA simultaneously
Outstanding performance across panel sizes, target regions, and multiplexing requirements
Easily add or enhance panel content
Unlocking the Complexity of Methylation

Hear from the Twist Product Manager and an NEB Application Scientist as they provide an overview of the NEB-to-Twist workflow. Learn the advantages of enzymatic conversion, the benefits of the Twist pre-capture conversion probe design approach, and the workflow's applicability to clinically relevant sample types.

Watch the Webinar 
 

 

Tools to Improve Methyl-seq Efficiency for Better Resolution in Epigenetic Studies

Dive deeper into Twist Bioscience's new Methyl-seq workflow built in partnership with New England Biolabs. Learn how it leads to increased confidence in methylated-base calls.

Read the Blog

*sold seperately

Library Prep
NEBNext® EM-seq™ Kit for Twist Targeted Methylation Sequencing

In partnership with New England Biolabs (NEB), Twist Bioscience offers a new methylation sequencing workflow that improves the quality of libraries and removes the need for damaging bisulfite treatment during prep. 
 
The workflow features enzymatic conversion of unmethylated cytosines (see figure) to identify sites of methyl-cytosine (5mC) and hydroxymethyl-cytosine (5hmC). 
 
Enzymatic conversion produces more intact libraries with better representation, and ultimately achieves more sensitive methylation detection. The library preparation system is suitable for whole genome sequencing and downstream enrichment with Twist Methylation Panels. 

EM-seq conversion involves a series of enzymatic steps to convert unmethylated cytosines into uracils. The final result is the same as conventional bisulfite conversion, making EM-seq compatible with existing analysis pipelines that use Bismark and bwa-meth. 

EM-seq conversion
NEBNext® EM-seq™ Kit for Twist Targeted Methylation Sequencing

In partnership with New England Biolabs (NEB), Twist Bioscience offers a new methylation sequencing workflow that improves the quality of libraries and removes the need for damaging bisulfite treatment during prep. 
 
The workflow features enzymatic conversion of unmethylated cytosines (see figure) to identify sites of methyl-cytosine (5mC) and hydroxymethyl-cytosine (5hmC). 
 
Enzymatic conversion produces more intact libraries with better representation, and ultimately achieves more sensitive methylation detection. The library preparation system is suitable for whole genome sequencing and downstream enrichment with Twist Methylation Panels. 

EM-seq conversion involves a series of enzymatic steps to convert unmethylated cytosines into uracils. The final result is the same as conventional bisulfite conversion, making EM-seq compatible with existing analysis pipelines that use Bismark and bwa-meth. 

EM-seq conversion
Target Enrichment
Twist Fast Hybridization System and Custom Methylation Panels

The Twist Fast Hybridization and Wash Kit provides optimal performance and maximum flexibility. Reagents and steps can be optimized to tune downstream sequencing metrics and to reduce hands-on time and pipette use. 

Designed with a highly sophisticated algorithm, Twist Custom Methylation Panels capture targets with exceptional efficiency across a wide range of target sizes. Together, these components ensure best-in-class hybrid capture of custom regions of interest.

Hybridization Time
Twist Methylation Enhancer

Off-target capture frequently occurs in targeted methylation sequencing workflows because the conversion process reduces the sequence complexity of the sample during library preparation. 

To maximize the capture of post-conversion samples, the Twist Methylation Detection System features a proprietary blocker designed specifically for methylation detection. Called Methylation Enhancer, this reagent reduces off-target capture by as much as half.  
 

Methylation Enhancer
Highly Sensitive Detection of Differentially Methylated Regions

Methylation levels vary substantially across the human genome, and differentially methylated regions (DMRs) can be used to identify certain cancers. 

To test the effects of methylation levels on the performance of the Twist Methylation Detection System, libraries of varying methylation levels (0-100% methylation) were generated by combining hypo- and hypermethylated genomic DNA in defined ratios. This analysis showed minimal effects of methylation level on final sequencing metrics.

The Twist Methylation Detection System also captures hypo- and hypermethylated regions with high sensitivity.

Highly Sensitive Detection of Differentially Methylated Regions
Twist Fast Hybridization System and Custom Methylation Panels

The Twist Fast Hybridization and Wash Kit provides optimal performance and maximum flexibility. Reagents and steps can be optimized to tune downstream sequencing metrics and to reduce hands-on time and pipette use. 

Designed with a highly sophisticated algorithm, Twist Custom Methylation Panels capture targets with exceptional efficiency across a wide range of target sizes. Together, these components ensure best-in-class hybrid capture of custom regions of interest.

Hybridization Time
Twist Methylation Enhancer

Off-target capture frequently occurs in targeted methylation sequencing workflows because the conversion process reduces the sequence complexity of the sample during library preparation. 

To maximize the capture of post-conversion samples, the Twist Methylation Detection System features a proprietary blocker designed specifically for methylation detection. Called Methylation Enhancer, this reagent reduces off-target capture by as much as half.  
 

Methylation Enhancer
Highly Sensitive Detection of Differentially Methylated Regions

Methylation levels vary substantially across the human genome, and differentially methylated regions (DMRs) can be used to identify certain cancers. 

To test the effects of methylation levels on the performance of the Twist Methylation Detection System, libraries of varying methylation levels (0-100% methylation) were generated by combining hypo- and hypermethylated genomic DNA in defined ratios. This analysis showed minimal effects of methylation level on final sequencing metrics.

The Twist Methylation Detection System also captures hypo- and hypermethylated regions with high sensitivity.

Highly Sensitive Detection of Differentially Methylated Regions
ORDERING
PRODUCT SKUS

101976

NEBNext® EM-seq™ Kit for Twist Targeted Methylation Sequencing, 96 Samples

103557

Twist Methylation Enhancer, 12 Reactions

100578

Twist Universal Blocker, 12 Reactions

100983

Twist Binding and Purification Beads Kit, 12 Reactions

101174

Twist Fast Hybridization and Wash Kit, 12 Reactions

103496

Twist Targeted Methylation Sequencing Workflow, 96x12 Reactions (contains all reagents listed above)

2 reaction and 12 reaction sizes also available for enrichment.

Twist Custom Panels can be ordered separately. Please contact your sales representative for more information.
 

PRODUCT SKUS

101976

NEBNext® EM-seq™ Kit for Twist Targeted Methylation Sequencing, 96 Samples

103557

Twist Methylation Enhancer, 12 Reactions

100578

Twist Universal Blocker, 12 Reactions

100983

Twist Binding and Purification Beads Kit, 12 Reactions

101174

Twist Fast Hybridization and Wash Kit, 12 Reactions

103496

Twist Targeted Methylation Sequencing Workflow, 96x12 Reactions (contains all reagents listed above)

2 reaction and 12 reaction sizes also available for enrichment.

Twist Custom Panels can be ordered separately. Please contact your sales representative for more information.
 

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