Epigenetic Analysis
The Twist NGS Methylation Detection System provides a robust, end-to-end sample preparation solution for identifying methylated regions in the human genome. The workflow employs a unique enzymatic process from New England Biolabs® that is much less damaging to DNA, alongside Twist‘s Custom Methylation Panel design.
Whether you are investigating cellular differentiation or screening liquid biopsies for cancer, the system offers the most efficient methylation detection available.
*sold seperately
Hear from the Twist Product Manager and an NEB Application Scientist as they provide an overview of the NEB-to-Twist workflow. Learn the advantages of enzymatic conversion, the benefits of the Twist pre-capture conversion probe design approach, and the workflow's applicability to clinically relevant sample types.
Dive deeper into Twist Bioscience's new Methyl-seq workflow built in partnership with New England Biolabs. Learn how it leads to increased confidence in methylated-base calls.
The Twist NGS Methylation Detection System provides a robust, end-to-end sample preparation solution for identifying methylated regions in the human genome. The workflow employs a unique enzymatic process from New England Biolabs® that is much less damaging to DNA, alongside Twist‘s Custom Methylation Panel design.
Whether you are investigating cellular differentiation or screening liquid biopsies for cancer, the system offers the most efficient methylation detection available.
Hear from the Twist Product Manager and an NEB Application Scientist as they provide an overview of the NEB-to-Twist workflow. Learn the advantages of enzymatic conversion, the benefits of the Twist pre-capture conversion probe design approach, and the workflow's applicability to clinically relevant sample types.
Dive deeper into Twist Bioscience's new Methyl-seq workflow built in partnership with New England Biolabs. Learn how it leads to increased confidence in methylated-base calls.
*sold seperately
In partnership with New England Biolabs (NEB), Twist Bioscience offers a new methylation sequencing workflow that improves the quality of libraries and removes the need for damaging bisulfite treatment during prep.
The workflow features enzymatic conversion of unmethylated cytosines (see figure) to identify sites of methyl-cytosine (5mC) and hydroxymethyl-cytosine (5hmC).
Enzymatic conversion produces more intact libraries with better representation, and ultimately achieves more sensitive methylation detection. The library preparation system is suitable for whole genome sequencing and downstream enrichment with Twist Methylation Panels.
EM-seq conversion involves a series of enzymatic steps to convert unmethylated cytosines into uracils. The final result is the same as conventional bisulfite conversion, making EM-seq compatible with existing analysis pipelines that use Bismark and bwa-meth.
In partnership with New England Biolabs (NEB), Twist Bioscience offers a new methylation sequencing workflow that improves the quality of libraries and removes the need for damaging bisulfite treatment during prep.
The workflow features enzymatic conversion of unmethylated cytosines (see figure) to identify sites of methyl-cytosine (5mC) and hydroxymethyl-cytosine (5hmC).
Enzymatic conversion produces more intact libraries with better representation, and ultimately achieves more sensitive methylation detection. The library preparation system is suitable for whole genome sequencing and downstream enrichment with Twist Methylation Panels.
EM-seq conversion involves a series of enzymatic steps to convert unmethylated cytosines into uracils. The final result is the same as conventional bisulfite conversion, making EM-seq compatible with existing analysis pipelines that use Bismark and bwa-meth.
The Twist Fast Hybridization and Wash Kit provides optimal performance and maximum flexibility. Reagents and steps can be optimized to tune downstream sequencing metrics and to reduce hands-on time and pipette use.
Designed with a highly sophisticated algorithm, Twist Custom Methylation Panels capture targets with exceptional efficiency across a wide range of target sizes. Together, these components ensure best-in-class hybrid capture of custom regions of interest.
Off-target capture frequently occurs in targeted methylation sequencing workflows because the conversion process reduces the sequence complexity of the sample during library preparation.
To maximize the capture of post-conversion samples, the Twist Methylation Detection System features a proprietary blocker designed specifically for methylation detection. Called Methylation Enhancer, this reagent reduces off-target capture by as much as half.
Methylation levels vary substantially across the human genome, and differentially methylated regions (DMRs) can be used to identify certain cancers.
To test the effects of methylation levels on the performance of the Twist Methylation Detection System, libraries of varying methylation levels (0-100% methylation) were generated by combining hypo- and hypermethylated genomic DNA in defined ratios. This analysis showed minimal effects of methylation level on final sequencing metrics.
The Twist Methylation Detection System also captures hypo- and hypermethylated regions with high sensitivity.
The Twist Fast Hybridization and Wash Kit provides optimal performance and maximum flexibility. Reagents and steps can be optimized to tune downstream sequencing metrics and to reduce hands-on time and pipette use.
Designed with a highly sophisticated algorithm, Twist Custom Methylation Panels capture targets with exceptional efficiency across a wide range of target sizes. Together, these components ensure best-in-class hybrid capture of custom regions of interest.
Off-target capture frequently occurs in targeted methylation sequencing workflows because the conversion process reduces the sequence complexity of the sample during library preparation.
To maximize the capture of post-conversion samples, the Twist Methylation Detection System features a proprietary blocker designed specifically for methylation detection. Called Methylation Enhancer, this reagent reduces off-target capture by as much as half.
Methylation levels vary substantially across the human genome, and differentially methylated regions (DMRs) can be used to identify certain cancers.
To test the effects of methylation levels on the performance of the Twist Methylation Detection System, libraries of varying methylation levels (0-100% methylation) were generated by combining hypo- and hypermethylated genomic DNA in defined ratios. This analysis showed minimal effects of methylation level on final sequencing metrics.
The Twist Methylation Detection System also captures hypo- and hypermethylated regions with high sensitivity.
101976
NEBNext® EM-seq™ Kit for Twist Targeted Methylation Sequencing, 96 Samples103557
Twist Methylation Enhancer, 12 Reactions100578
Twist Universal Blocker, 12 Reactions100983
Twist Binding and Purification Beads Kit, 12 Reactions101174
Twist Fast Hybridization and Wash Kit, 12 Reactions103496
Twist Targeted Methylation Sequencing Workflow, 96x12 Reactions (contains all reagents listed above)2 reaction and 12 reaction sizes also available for enrichment.
Twist Custom Panels can be ordered separately. Please contact your sales representative for more information.
101976
NEBNext® EM-seq™ Kit for Twist Targeted Methylation Sequencing, 96 Samples103557
Twist Methylation Enhancer, 12 Reactions100578
Twist Universal Blocker, 12 Reactions100983
Twist Binding and Purification Beads Kit, 12 Reactions101174
Twist Fast Hybridization and Wash Kit, 12 Reactions103496
Twist Targeted Methylation Sequencing Workflow, 96x12 Reactions (contains all reagents listed above)2 reaction and 12 reaction sizes also available for enrichment.
Twist Custom Panels can be ordered separately. Please contact your sales representative for more information.
Technical Note
Highly Sensitive Methylation Detection Using Enzymatic Methyl-seq and Twist Target Enrichment
Technical Note
Minimizing Off-Target Capture in Targeted Methyl-Seq Through Panel Design & Enhancer Reagents
Technical Note
Optimizing the Performance of the Twist Targeted Methylation Sequencing Workflow
Technical Note
Highly Sensitive Methylation Detection Using Enzymatic Methyl-seq and Twist Target Enrichment
Technical Note
Minimizing Off-Target Capture in Targeted Methyl-Seq Through Panel Design & Enhancer Reagents
Technical Note
Optimizing the Performance of the Twist Targeted Methylation Sequencing Workflow
