Capitalize on Proven Binding Motifs
Twist Ancestral scFv 文库是一种新的合成抗体文库，根据在一组精心设计且广泛应用的治疗和诊断抗体中观察到的趋势开发而来。通过捕获在所检测抗体序列中发现的多样性并模仿人抗体组库，该文库具有比原始文库更高质量的序列，可帮助您发现靶向任何靶标的更优抗体。
利用这个独特的文库，您可以发现和开发针对任何适应症的治疗性抗体。
Capitalize on Proven Binding Motifs
Twist Ancestral scFv 文库是一种新的合成抗体文库，根据在一组精心设计且广泛应用的治疗和诊断抗体中观察到的趋势开发而来。通过捕获在所检测抗体序列中发现的多样性并模仿人抗体组库，该文库具有比原始文库更高质量的序列，可帮助您发现靶向任何靶标的更优抗体。
利用这个独特的文库，您可以发现和开发针对任何适应症的治疗性抗体。
The Twist Ancestral scFv Library leverages information from 22,426 existing human, monkey, and alpaca antibodies. The library excludes potential development liability motifs while selecting for optimal complementarity determining region (CDR) sequences and lengths that generate the diversity of this antibody set. The heavy chain library permutates CDR sequences from 100 unique CDR1s, 100 unique CDR2s, and 845 unique CDR3s within the human IGHV3-23 framework. The light chain library assembles diverse combinations of CDRs from 80 unique CDR1s, 80 unique CDR2s, and 400 unique CDR3s within the human IGKVK1-39 framework. When combined, the assembled heavy and light chain libraries yield a fully human scFv library with a diversity of 1 x 109.
The Twist Ancestral scFv Library leverages information from 22,426 existing human, monkey, and alpaca antibodies. The library excludes potential development liability motifs while selecting for optimal complementarity determining region (CDR) sequences and lengths that generate the diversity of this antibody set. The heavy chain library permutates CDR sequences from 100 unique CDR1s, 100 unique CDR2s, and 845 unique CDR3s within the human IGHV3-23 framework. The light chain library assembles diverse combinations of CDRs from 80 unique CDR1s, 80 unique CDR2s, and 400 unique CDR3s within the human IGKVK1-39 framework. When combined, the assembled heavy and light chain libraries yield a fully human scFv library with a diversity of 1 x 109.
从淘选到功能分析，大概需要 10-12 周。The process starts with phage screening the diverse Twist Ancestral scFv Library against target antigens and ends with reformatting candidate antibody fragments to full-length IgG.
You can also license the Ancestral scFv library to initiate your own in-house discovery projects. 欲了解详情，请联系 [email protected]。
从淘选到功能分析，大概需要 10-12 周。The process starts with phage screening the diverse Twist Ancestral scFv Library against target antigens and ends with reformatting candidate antibody fragments to full-length IgG.
You can also license the Ancestral scFv library to initiate your own in-house discovery projects. 欲了解详情，请联系 [email protected]。
概念验证数据
The Twist Ancestral scFv Library was successfully panned against SARS-CoV-2 Spike Protein S1. A large number of unique clones with diverse binding affinities were identified.
Kinetics with directly coupled anti-S1 antibodies via surface plasmon resonance identifies antibodies like TB212-12 and TB212-56 with high binding affinity for S1 and S trimer.
Flow titration demonstrates that TB212-12 and TB212-56 show inhibition of S1 binding to ACE2-expressing VERO E6 cell
概念验证数据
The Twist Ancestral scFv Library was successfully panned against SARS-CoV-2 Spike Protein S1. A large number of unique clones with diverse binding affinities were identified.
Kinetics with directly coupled anti-S1 antibodies via surface plasmon resonance identifies antibodies like TB212-12 and TB212-56 with high binding affinity for S1 and S trimer.
Flow titration demonstrates that TB212-12 and TB212-56 show inhibition of S1 binding to ACE2-expressing VERO E6 cell
